Using alkaline phosphatase (AP) as an enzymatic label can help you achieve lower limits of detection. With the electroactive enzymatic products obtained from p-aminophenyl phosphate, hydroquinone diphosphate, and phosphorylated paracetamol, you can achieve simple electrochemical detection while increasing sensitivity and getting wider linear ranges than would be obtained with other standard AP substrates.
Oxidation potentials of the enzymatic products are lower than those of other AP products, thus reducing the number of potential interferents able to be oxidized at the electrode surface.
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